Increasing the Efficiency of Homology-Directed Repair for CRISPR-Cas9-Induced Precise Gene Editing in Mammalian Cells

What Baby KJ means for the CRISPR gene editing industry

The custom-built gene editing treatment for 6-month-old KJ Muldoon could not have come at a more welcome or jarring time for CRISPR-powered biomedicine.

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway. The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared with the higher efficiency of the nonhomologous end-joining (NHEJ) pathway. To enhance HDR, enabling the insertion of precise genetic modifications, …

Gene-Editing Breakthrough Saves an Infant with a Rare Disease

CRISPR therapies are moving from proof-of-concept to real-world treatments for a growing list of diseases.

CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing

The development of a method for genome editing based on CRISPR–Cas9 technology was awarded The Nobel Prize in Chemistry in 2020, less than a decade a

Small molecule makes RDEB gene editing more accurate: Study

Researchers used a small molecule called M3814 for more accurate editing of genetic mutations causing recessive dystrophic epidermolysis bullosa (RDEB), according to a new study. This method enabled skin cells from three patients to restore their production of type VII collagen (C7) protein, which they lacked, the researchers noted. M3814 works by inhibiting DNA-dependent protein kinase, an enzyme that repairs breaks in DNA. Using M3814, the res…